Composite

Part:BBa_K2959001

Designed by: Alejandro Aguirre Hernndez   Group: iGEM19_Tec-Chihuahua   (2019-08-26)
Revision as of 21:34, 27 August 2019 by Alejandro ah (Talk | contribs)


Expressible LL 37 with Arabinose Inducible Promoter

This composite part enables tha expression of the human antimicrobial peptide LL 37. The BioBrick consists of parts araC-pBad-rbs-LL37 (BBa_K1230007) and a doulbe terminator (BBa_B0015). The part was designed with the purpose of expressing the peptide in E. coli. Expression of the part is regulated by the pBAD promoter which is inducible through arabinose. The promoter was designed to have low background activty which is ideal for porduction of toxic peptides which is the case of LL 37 production in E. coli. Also, we added part BBa_B0015 in order to create a ready-to-express version of part BBa_K1230007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None