Measurement

Part:BBa_K2909004

Designed by: Laurent CHEN   Group: iGEM19_Sorbonne_U_Paris   (2019-08-27)
Revision as of 15:31, 27 August 2019 by Laurent CHEN (Talk | contribs)


ParoR_CloverGFP

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal PstI site found at 2806
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal NheI site found at 269
    Illegal PstI site found at 2806
    Illegal NotI site found at 1779
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal BamHI site found at 11
    Illegal BamHI site found at 3413
    Illegal XhoI site found at 2320
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal PstI site found at 2806
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal PstI site found at 2806
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3451


Introduction

1- Biological background

This part is a reporter construct with a Clover GFP reporter gene under the control of an inducible PNIT promoter.

2- Usage in iGEM projects

Bio[oil]gical Factory (iGEM Sorbonne Université 2019)

Characterization

References

  1. Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
  2. 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).
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