Composite

Part:BBa_K3182300

Designed by: Oliver Hild Walett   Group: iGEM19_Linkoping_Sweden   (2019-07-15)
Revision as of 07:57, 27 August 2019 by Emmbe580 (Talk | contribs)


pT7-mNG

mNeonGreen with a T7 promotor.

Figure 3. Photo of this mNG part (BBa_K3182300) after 16h of induction at 16°C over-night. It was expressed in BL21 (DE3) gold with 1mM IPTG. To the right of mNG are mTFP expressed in BL21 and V. Natriegens (Vmax strain).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 91
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None