Coding

Part:BBa_K2973009

Designed by: Nick Delkis, Aphrodite Katsaouni   Group: iGEM19_Thessaly   (2019-08-26)
Revision as of 21:57, 26 August 2019 by Registry (Talk | contribs)

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IS6110 (Mycobacterium tuberculosis)

This basic part is the coding sequence of the Mycobacterium tuberculosis (MTB) IS6110 gene. IS6110 is an insertion element found exclusively within the members of the Mycobacterium tuberculosis complex (MTBC), and because of this exclusivity, it has become an important diagnostic tool in the identification of MTBC species. The restriction of IS6110 to the MTBC is hypothesized to arise from the inability of these bacteria to exchange DNA. The gene IS6110 encodes for proteins responsible for the transposition activity that occurs naturally in a wide variety of organisms. IS6110 is scattered four to 25 times throughout the MTB genome.

For these reasons, IS6110 is commonly used as a DNA biomarker for the detection of MTB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 880
    Illegal XhoI site found at 1266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 232
    Illegal NgoMIV site found at 1305
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1314
    Illegal BsaI.rc site found at 1295


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Categories
Parameters
None