Part:BBa_K2909003

DGAT-1-2-B3-B4 E. guineensis codon optimized for C. reinhardtii

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 162
  Illegal PstI site found at 1590
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 162
  Illegal PstI site found at 1590
  Illegal NotI site found at 400
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1498
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 162
  Illegal PstI site found at 1590
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 162
  Illegal PstI site found at 1590
  Illegal NgoMIV site found at 531
  Illegal NgoMIV site found at 624
  
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

1- Biological background

Coding sequence of the DGAT-1-2 enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.

It catalyzes the incorporation of the third and last fatty acid at the position sn-3 of the glycerol in the triglyceride synthesis (Kennedy) pathway.

The CDS is standardized to the C. reinhardtii MoClo Kit for the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends.

The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.

2- Usage in iGEM projects

Bio[oil]gical Factory (iGEM Sorbonne Université 2019)

References

1. Dussert, S. et al. Comparative Transcriptome Analysis of Three Oil Palm Fruit and Seed Tissues That Differ in Oil Content and Fatty Acid Composition. PLANT PHYSIOLOGY 162, 1337–1358 (2013).
2. Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
3. 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).