Coding

Part:BBa_K2909003

Designed by: Laurent CHEN   Group: iGEM19_Sorbonne_U_Paris   (2019-08-26)
Revision as of 16:17, 26 August 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


DGAT-1-2-B3-B4 E. guineensis codon optimized for C. reinhardtii

Coding sequence of the LPAAT-A enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.

It catalyzes the incorporation of the third and last fatty acid at the position sn-3 of the glycerol in the triglyceride synthesis (Kennedy) pathway.

The CDS is standardized to the C. reinhardtii MoClo Kit for the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 162
    Illegal PstI site found at 1590
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 162
    Illegal PstI site found at 1590
    Illegal NotI site found at 400
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1498
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 162
    Illegal PstI site found at 1590
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 162
    Illegal PstI site found at 1590
    Illegal NgoMIV site found at 531
    Illegal NgoMIV site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None