Coding

Part:BBa_K2909002

Designed by: Laurent CHEN   Group: iGEM19_Sorbonne_U_Paris   (2019-08-26)
Revision as of 16:12, 26 August 2019 by Laurent CHEN (Talk | contribs)

LPAAT-A-B3-B4 E. guineensis codon optimized for C. reinhardtii

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 474
    Illegal NgoMIV site found at 204
    Illegal NgoMIV site found at 1029
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

1- Biological background

Coding sequence of the LPAAT-A enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.
It catalyzes the incorporation of the second fatty acid at the position sn-2 of the glycerol in the triglyceride synthesis (Kennedy) pathway.
The CDS is standardized to the C. reinhardtii MoClo Kit for the B3-B4 position.

2- Usage in iGEM projects

Bio[oil]gical Factory (iGEM Sorbonne Université 2019)

Characterization

References

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Categories
Parameters
None