Tag

Part:BBa_K2909000

Designed by: Laurent CHEN   Group: iGEM19_Sorbonne_U_Paris   (2019-08-26)
Revision as of 15:08, 26 August 2019 by Laurent CHEN (Talk | contribs)

HiBiT-B2 MoClo C. reinhardtii

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

1- Biological background

HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence (https://www.promega.com/resources/pubhub/features/hibit-a-tiny-tag-for-antibody-free-endogenous-protein-detection/). This part is standardized in the MoClo standard for Chlamydomonas reinhardtii. This tag is flanked on both side by specific fusion sites and BbsI sites to allow its integration into a level 0 plasmid of the C. reinhardtii MoClo Kit. This tag is designed to be integrated at the B2 position (N-terminal tag).

2- Usage in iGEM projects

Bio[oil]gical Factory (iGEM Sorbonne Université 2019)

Characterization

References

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Categories
Parameters
None