Tag
Part:BBa_K2909000:Design
Designed by: Laurent CHEN Group: iGEM19_Sorbonne_U_Paris (2019-08-26)
Revision as of 14:41, 26 August 2019 by Laurent CHEN (Talk | contribs)
Design Notes
We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018). BbsI sites and fusion sites corresponding to the B2 position (N-terminal tag) were added on both side to be compatible with the MoClo assembly standard.
Source
This part comes from Promega (Schwinn et al. 2018).
References
Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018). Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).