Tag

Part:BBa_K2909000

Designed by: Laurent CHEN   Group: iGEM19_Sorbonne_U_Paris   (2019-08-26)
Revision as of 14:05, 26 August 2019 by Registry (Talk | contribs)

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HiBiT-B2 MoClo C. reinhardtii

HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence. This part is standardized in the MoClo standard for Chlamydomonas reinhardtii. This tag is flanked on both side by specific fusion sites and BsaI sites to allow its integration into a level 0 plasmid of the C. reinhardtii MoClo Kit. This tag is designed to be integrated at the B2 position (N-terminal tag).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None