Composite

Part:BBa_K2973002:Design

Designed by: Nick Delkis   Group: iGEM19_Thessaly   (2019-08-17)
Revision as of 16:32, 17 August 2019 by Tkontogiannis (Talk | contribs) (→‎References)

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T7-RBS- enhanced green fluorescent protein (EGFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 776
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added double stop codon TAA at the end of the CDS.


Source

The EGFP CDS was taken from the part BBa_K1094400

References

Zhang, Guohong, et al. “An Enhanced Green Fluorescent Protein Allows Sensitive Detection of Gene Transfer in Mammalian Cells.” Biochemical and Biophysical Research Communications, vol. 227, no. 3, 1996, pp. 707–711., doi:10.1006/bbrc.1996.1573.