Coding

Part:BBa_K2973001:Design

Designed by: Thodoris Kontogiannis   Group: iGEM19_Thessaly   (2019-08-17)
Revision as of 16:24, 17 August 2019 by Tkontogiannis (Talk | contribs) (→‎References)

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β-Lactamase-No-signal-peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to delete the signal peptide from the N-terminal end of b-lactamase in order to do our in vitro experiments.


Source

NCBI Reference Sequence: WP_000027057.1

References

Qureshi, Sohail A. “β-Lactamase: an Ideal Reporter System for Monitoring Gene Expression in Live Eukaryotic Cells.” BioTechniques, vol. 42, no. 1, 2007, pp. 91–96., doi:10.2144/000112292.

Boehle, Katherine E., et al. “Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics.” ACS Sensors, vol. 3, no. 7, 2018, pp. 1299–1307., doi:10.1021/acssensors.8b00163