Composite
Part:BBa_K2949012
Designed by: Yujie Wang Group: iGEM19_AHUT_China (2019-08-12)
Carbonic Anhydrase 2(L203K)-N-LCTASR
This part is the coding sequence (CDS) of the N-terminal modified Carbonic anhydrase 2(L203K)(BBa_K2547004).Formylglycine generating enzyme can selectively recognize and oxidize the cysteine residues in the sulfatase subunit of the protein terminal to form formylglycine (FGly) residues containing aldehyde groups.The terminal aldehyde group of CA2, is fixed to surface active carriers containing amino groups by covalent binding. This site-specific fixation avoids the chemical degeneration that may occur in common covalent fixation and has higher stability than physical adsorption.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |