Coding

Part:BBa_K2982000:Design

Designed by: Tsang Hoi Yeung, Ocean   Group: iGEM19_HK_GTC   (2019-08-03)
Revision as of 04:37, 7 August 2019 by T ocean (Talk | contribs) (→‎References)

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Coding sequence for wild type IsPETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 348
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Extra amino acids are added to the N-terminus and C-terminus of the codon optimized DNA sequence used for expression of wild type Ideonella sakainesis PETase.


Source

Sequence of wild type PETase optimized for Escherichia coli from 'Characterization and engineering of a plastic-degrading aromatic polyesterase', PNAs, 2018.

References

Austin, H. P., Allen, M. D., Donohoe, B. S., Rorrer, N. A., Kearns, F. L., Silveira, R. L., . . . Beckham, G. T. (2018). Characterization and engineering of a plastic-degrading aromatic polyesterase. Proceedings of the National Academy of Sciences, 115(19). doi:10.1073/pnas.1718804115