Tag
Part:BBa_K2976008
Designed by: Jiatong Chen Group: iGEM19_CPU_CHINA (2019-08-04)
Revision as of 00:44, 5 August 2019 by NF-kB pathway (Talk | contribs)
Usage and Biology
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates the following characteristics: solubility, detection, purification, localization and expression. The HA tag (YPYDVPDYA) is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors due to its small size and low influence on the tagged protein’s biochemical properties. In 2019 CPU_CHINA project, we insert HA tag into the fusion proteins on the membrane of exosomes for the immunoblotting assay.
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