Coding

Part:BBa_K2982003

Designed by: Tsang Hoi Yeung, Ocean   Group: iGEM19_HK_GTC   (2019-08-03)
Revision as of 13:01, 3 August 2019 by Registry (Talk | contribs)

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Coding sequence for W159H/S214H IsPETase double mutant

A coding sequence for mutated W159H/S214H PETase from Ideonella sakainesis which is in BioBrick RFC[10] assembly standard and also contains two site for restriction enzymes, XhoI and BamHI to act on. It is codon optimized for Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 348
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None