Coding
PheRS

Part:BBa_K2916007

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-24)
Revision as of 13:31, 24 July 2019 by Konstantinos (Talk | contribs)


PheRS protein equipped with a 6x HIS affinity tag

Glycyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.This protein includes both GlyRS-alpha and GlyRS-beta domains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2424
    Illegal BamHI site found at 31
    Illegal BamHI site found at 1772
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1162
    Illegal AgeI site found at 1155
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2929
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2875
    Illegal SapI.rc site found at 198
    Illegal SapI.rc site found at 2851


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Categories
Parameters
None