Coding
PheRS

Part:BBa_K2916007:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-24)
Revision as of 13:24, 24 July 2019 by Registry (Talk | contribs)

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PheRS protein equipped with a 6x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2424
    Illegal BamHI site found at 31
    Illegal BamHI site found at 1772
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1162
    Illegal AgeI site found at 1155
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2929
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2875
    Illegal SapI.rc site found at 198
    Illegal SapI.rc site found at 2851


Design Notes

The sequence was extracted from the pET21a-GlyRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.


Source

http://www.addgene.org/124110/

References