Coding
LysRS

Part:BBa_K2916006:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-24)
Revision as of 13:08, 24 July 2019 by Registry (Talk | contribs)

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LysRS protein equipped with a 6x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1121
    Illegal XhoI site found at 1516
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475


Design Notes

The sequence was extracted from the pET21a-GluRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.


Source

http://www.addgene.org/124109/

References