Coding
GlyRS

Part:BBa_K2916003:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-23)
Revision as of 15:55, 23 July 2019 by Konstantinos (Talk | contribs)


GlnRS protein equipped with a 6x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 54
    Illegal BglII site found at 1002
    Illegal XhoI site found at 1663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1608
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was extracted from the pET16b-His-ArgRS plasmid and expressed in the BL21 (DE3) competent E.coli strain

Source

http://www.addgene.org/124104/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568