Coding
ArgRS

Part:BBa_K2916000:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-19)
Revision as of 14:58, 23 July 2019 by Konstantinos (Talk | contribs) (→‎Design Notes)


ArgRS protein equipped with a 10x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 76
    Illegal SapI.rc site found at 1495


Design Notes

The sequence was extracted from the pQE30-His-AlaRS plasmid and expressed in the M15 E.coli strain

Source

Escherichia coli gene alaS

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568