Reporter
sfGFP

Part:BBa_K3182008

Designed by: Oliver Hild Walett   Group: iGEM19_Linkoping_Sweden   (2019-07-14)
Revision as of 13:41, 23 July 2019 by Emmbe580 (Talk | contribs)

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sfGFP

sfGFP with BamHI recognition site added in the start.

Figure 1. General mechanism of action when the antimicrobial agent is fused to BBa_K3182010 which contains the linker, thrombin site and cellulose binding domain. The cellulose binding domain enables the attachment of antimicrobial agents (such as antimicrobial peptide or enzyme) to the cellulose. This also inactivates the agents when they are expressed in its bacterial host. Later, when the bandage comes in contact with thrombin in the blood, the antimicrobial agents will be activated upon cleavage of its thrombin site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


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