Regulatory

Part:BBa_J119443

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2019-07-18)
Revision as of 20:56, 18 July 2019 by Eckdahl (Talk | contribs)


RET Promoter G-quadruplex and i-Motif

The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The G-quadruplex is shown on the coding strand in this sequence. The i-motif/G-quadruplex-forming sequence comes from the human RET promoter. The G-quadruplex is shown on the coding strand of this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, however, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None