Regulatory

Part:BBa_K2967002:Design

Designed by: Rui Wang   Group: iGEM19_NEU_CHINA   (2019-07-17)
Revision as of 15:59, 17 July 2019 by Wangrui (Talk | contribs) (→‎References)

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a 54 -dependent hrpL promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of the protein complex HrpRS specifically acting on the hrpL promoter region has not been investigated yet, so the hrpL promoter region in the amplifier is relatively long. In order to ensure that the transcription initiation is completed normally, we do not change the sequence.


Source

it is from the hrp (hypersensitive response and pathogenicity) gene regulatory network for Type III secretion in Pseudomonas syringae.

References

Wang,B., Kitney,R.I., Joly,N. and Buck,M. (2011) Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology. Nat. Commun., 2, 508.