Protein_Domain
CHAP

Part:BBa_K3182004

Designed by: Leo Juhlin   Group: iGEM19_Linkoping_Sweden   (2019-07-13)
Revision as of 15:05, 15 July 2019 by Emmbe580 (Talk | contribs)


CHAP(K)

Staphylococcus phage endolysin, designed to target Staphylococcus aureus. This part was designed to be assembled with BBa_K3182010.

Figure 1. General mechanism of action when the antimicrobial agent is fused to BBa_K3182010 which contains the linker, thrombin site and cellulose binding domain. The cellulose binding domain enables the attachment of antimicrobial agents (such as antimicrobial peptide or enzyme) to the cellulose. This also inactivates the agents when they are expressed in its bacterial host. Later, when the bandage comes in contact with thrombin in the blood, the antimicrobial agents will be activated upon cleavage of its thrombin site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None