Composite

Part:BBa_K3182000

Designed by: Oliver Hild Walett   Group: iGEM19_Linkoping_Sweden   (2019-07-08)
Revision as of 17:09, 11 July 2019 by Olle111 (Talk | contribs)


pT7-CBDcipA-AsPink

A cellulose binding domain (CBD) which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added at the end, clevage with thrombin will add one glycine and one serine to the start of the C-terminal of the fusion tail. The part has a AsPink fused to the CBD to ensure easy trackable expression and characterization of the CBD. The part has a very strong expression with a T7 promotor as well as a 5'UTR region which has been shown to further increase expression in E. coli.

Figure 1. E. coli BL21 cells expressing this biobrick, incubated for 16 hours at 16 degrees Celsius.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 580
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None