Composite

Part:BBa_K2905013:Design

Designed by: Brianna Ricker   Group: iGEM19_Alma   (2019-06-24)
Revision as of 19:30, 24 June 2019 by Briannaricker (Talk | contribs) (→‎Source)

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Constitutively expressed 6-His-TEV-PylS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized; 3A assembly scar sites included between promoter, RBS, and coding region.

Source

IDT synthesized DNA.

References