Composite

Part:BBa_K2905001:Design

Designed by: Devin Camenares   Group: iGEM19_Alma   (2019-06-24)
Revision as of 18:30, 24 June 2019 by Briannaricker (Talk | contribs) (→‎Design Notes)


Constitutively expressed 6-His-TEV-MttB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 104
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1220
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 869
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1046


Design Notes

Codon optimized; 3A assembly scar sites included between J23101, RBS, and coding region.

Source

IDT synthesized DNA.

References