Composite

Part:BBa_K2905009:Design

Designed by: Brianna Ricker   Group: iGEM19_Alma   (2019-06-24)
Revision as of 18:10, 24 June 2019 by Registry (Talk | contribs)

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Constitutively expressed 6-His-TEV-PylC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 214
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized. 3A assembly scar sites included between J23101, RBS, and Coding region.


Source

IDT synthesized DNA, codon optimized.

References