Composite
Part:BBa_K2905005:Design
Designed by: Brianna Ricker Group: iGEM19_Alma (2019-06-24)
Constitutively expressed 6-His-TEV-MtbA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 104 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized. 3A assembly scar sites included between J23101, RBS, and Coding region.
Source
IDT synthesized DNA, codon optimized.