Part:BBa_K2976000
Toll-like receptor 1
Toll-like receptor 1 (TLR1) is a transmembrane glycoprotein that participates in the innate immune response to microbial agents. TLR1 could form active heterodimers with TLR2 when exposed to some pathogen-associated molecular pattern molecules (PAMPs), and the heterodimers recognize plenty of substances in lipoarabinomannan (LAM) biosynthesis with the help of CD14.
Usage
In 2019 CPU_CHINA project, TLR1 is expressed along with TLR2 and CD14 to form the TLR2:TLR1:CD14 cluster on the designer cell membrane. As a Mtb sensor, the complex could recognize the substances of Mtb and then stimulate the downstream signaling pathway. Then, activated NF-κB initiates transcription of the gene circuits to express other proteins in our project.
Biology
After being activated with Mtb, the activation cluster TLR2:TLR1:CD14 triggers NF-kappa-B signaling pathways via MYD88 and TRAF6. NF-κB proteins exist in the cytoplasm in an inactive form because of their association with the IκB proteins. IκB proteins mask the nuclear-localization sequences (NLSs) of NF-κB subunits and retain it in the cytoplasm. Activation of TLR2:TLR1:CD14 cluster cause the degradation of IκB proteins by proteasomes. Then, NF-κB subunits could pass through the nuclear pore complex (NPC) and cause the expression of an array of pro-inflammatory cytokines and chemokines. Similarly, NF-κB subunits can also bind the NF-κB induced promoter and initiate transcription of the downstream genes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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