Composite

Part:BBa_M50499

Designed by: Teaghan Cowles, Rishabh Kapoor, Alex Bradfield, Yash Pershad   Group: Stanford BIOE44 - S11   (2018-10-21)
Revision as of 23:39, 11 December 2018 by TRAY (Talk | contribs)


Catabolite Activator Protein (CAP) Repressed Promoter

We placed the constitutive promoter (BBa_S05450, iGEM) immediately upstream from a CAP binding site (BBa_M36547, iGEM) in Plasmid E.

For our experiments, we placed this part upstream from GFP in order to assay expression levels through fluorescent intensity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None