Composite

Part:BBa_M50499:Design

Designed by: Teaghan Cowles, Rishabh Kapoor, Alex Bradfield, Yash Pershad   Group: Stanford BIOE44 - S11   (2018-10-21)
Revision as of 02:12, 22 October 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Catabolite Activator Protein (CAP) Repressed Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1059
    Illegal PstI site found at 688
    Illegal PstI site found at 849
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were unsure whether to put the CRP binding site immediately downstream or with a buffer sequence. We ended up putting it right after the promoter.


Source

IGEM part: BBa_S05450 = constitutive promoter IGEM part: BBa_M36547 = CRP binding site

Paper: www.ncbi.nlm.nih.gov/pmc/articles/PMC338411/

References