Part:BBa_K2787014
GFP under C9 orthogonal RBS with orthogonal 16S rRNA BBa_K2787042
The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSC9-GFP(BBa_K2787002) are linked into one plasmid to quantify the orthogonality of the TJU and C9 ribosome system.
Usage and Biology
A plasmid under control of C9 orthogonal RBS is constructed with the TJU orthogonal ribosome.
Expression of the target gene under the control of different orthogonal RBS with different orthogonal ribosomes are tested.
Expression of target gene under control of different RBSs with TJU orthogonal ribosome is tested, showing that the TJU ribosome is orthometric to other RBSs.
Expression of the target gene under control of the C9 orthogonal RBS with different orthogonal ribosomes to test the orthogonality of the C9 orthogonal RBS with other ribosomes. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 798
Illegal NheI site found at 821 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1661
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1675
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 707
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