Part:BBa_K2787012
GFP under A2 orthogonal RBS with orthogonal 16S rRNA BBa_K2787040
The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to quantify the orthogonality of the TJU and A2 ribosome system.
Usage and Biology
A plasmid with GFP under control of the A2 orthogonal RBS with the TJU orthogonal ribosome on it is constructed.
The GFP under control of A2 orthogonal RBS is tested within different 16s rRNAs. The fluorescence with A2 orthogonal ribosome is the best among host ribosomes and A2,B8,C9 ribosomes, which shows the good orthogonality of A2 16s rRNA with A2 orthogonal RBS.
The fluorescence under the test with 3 different orthogonal RBS and 3 different orthogonal ribosmes. It shows that the orthogonality of A2 ribosome and A2 RBS is one of the best ones.
The GFP under control of different orthogonal RBS is tested within TJU 16s rRNAs. There is not huge differences between the fluorescence with different orthogonals. It shows that the B8 orthogonal RBS is orthogonal to A2,TJU and C9 16s ribosomes. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 797
Illegal NheI site found at 820 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1660
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1674
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 706
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