Part:BBa_K2700001
Antisense-RNA for transcriptional/translational repression
This is our pt181-antisense-RNA transcript that will block both transcription and translation at the pt181 promoter (Part:BBa_K2700002) site. Once pt181-antisense is expressed, it will bind to the promoter region and cause a physical fold to occur. This fold is a stem-like structure that will block the RBS as well as act as a terminator to prevent further transcription to occur.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
pT181-antisense The pT181-antisense transcript interacts with the 5’ untranslated region of the pT181-promoter, inducing a conformational chance leading to transcriptional and translation repression, as seen in figure 2 [2].
1. Novick, R.P., et al., pT181 plasmid replication is regulated by a countertranscript-driven transcriptional attenuator. Cell, 1989. 59(2): p. 395-404. 2. Westbrook, A.M. and J.B. Lucks, Achieving large dynamic range control of gene expression with a compact RNA transcription–translation regulator. Nucleic Acids Research, 2017. 45(9): p. 5614-5624.
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