Composite

Part:BBa_K2868024:Design

Designed by: Cale Lester   Group: iGEM18_Stanford-Brown-RISD   (2018-10-09)
Revision as of 03:09, 18 October 2018 by Cale (Talk | contribs) (→‎Design Notes)

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csgA:CBD Expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was designed such that it will express the fusion protein we designed, csgA-CBD. The promoter is a constitutive T7 promoter which requires T7 RNA polymerase to be expressed. The coding region is the curlin precursor protein from E.Coli csgA linked to the chitin binding domain of Bacillus circulans, with a 6x polyhistidine tag on the end. As csgA has well documented amyloid forming behavior we theorized that the fusion protein with a chitin binding domain would make an exceptional biological adhesive for use on a mycelium substrate.

Source

Chitin binding domain from NEB, csgA is from E.Coli. See basic part pages for their sources.

References