Composite
Hly-OmpT

Part:BBa_K2588030:Design

Designed by: Daniel Wedemeyer   Group: iGEM18_Hamburg   (2018-10-05)
Revision as of 03:09, 18 October 2018 by BKlopprogge (Talk | contribs) (→‎Design Notes)

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Hly secretion system and OmpT generator, arabinose-induced


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1435
    Illegal BglII site found at 2098
    Illegal BamHI site found at 65
    Illegal BamHI site found at 4142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 365
    Illegal AgeI site found at 3602
    Illegal AgeI site found at 3659
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

non

Source

GGA-assembled composite of pBAD promoter (BBa_K206000), parts of TypeII secretion system BBa_K554013 by Brazil-France 2011, and Outer membrane protease OmpT amplified by PCR from E. coli genomic DNA.

References