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Part:BBa_K2719003:Design

Designed by: Karla Soto Blas   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 03:05, 18 October 2018 by AKARAM (Talk | contribs) (→‎References)

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GST (Optimized for E.coli BL21)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 346


Design Notes

The sequence of GST from Schistosoma japonicum, was optimized for its expression on E.coli BL21 (DE3). This part is useful, as by fussing a protein on the N-terminus, it improves its stability, solubility and allows its purification by affinity column.

"T--TecCEM--GST3DStructure.png"

Figure 1. GST 3D structure, modeled with Swiss-Model

Source

Synthetic part.

References

Harper S. & Speicher D. (2010). Purification of proteins fused to glutathione S-transferase. Protein Chromatography . Doi: 10.1007/978-1-60761-913-0_14.