Regulatory

Part:BBa_K2575001:Design

Designed by: Stephan George   Group: iGEM18_UGA   (2018-10-10)
Revision as of 02:59, 18 October 2018 by Sng82526 (Talk | contribs)

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6X UAS-35S minimal promoter Due to repetitive sequences, the part had to be cloned out of pJAB 1504 using primers to add iGEM restriction sites.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 208
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 208
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 208
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 208
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR out of vector, not able to be synthesized in 1 unit


Source

Synthetic/minimal promoter from cauliflower mosaic virus design

References