Regulatory

Part:BBa_K2876000:Experience

Designed by: Isaac Justice   Group: iGEM18_Stanford   (2018-06-22)
Revision as of 02:56, 18 October 2018 by Ijustice (Talk | contribs)

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To test the reporter we transformed DH5-alpha with the pOL2-62 + RFP reporter plasmid, as well as the positive control bacteriomatch lambda and alpha plasmids from Addgene (https://www.addgene.org/browse/article/8393/) and induced with IPTG. Unfortunately, when we ran the experiment on the plate reader we saw no fluorescence response (Figure 1).


RFPgraphs.png

Figure 1: 48hr plate reader fluorescence experiment of pOL2-62 + RFP reporter with bacteriomatch system (positive control lambda and alpha fusion proteins) and induced with IPTG.

Applications of BBa_K2876000

prokaryotic two hybrid systems

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