Part:BBa_K2588021

Growth inhibition module, DAP-repressed

The growth inhibition module inhibits E. coli cell division and growth. For further information visit our best composite part page.

Characterization

To characterize our growth inhibition module, we measured the optical density of the inhibited (+DAP), induced (-DAP), positive (DH5α) cultures at OD₆₀₀ nm for 6 hours at 1-hour intervals. The part was assembled using the Golden Gate assembly method. The growth inhibition module is regulated by a dapAP promoter, which is inhibited by the presence of diaminopimelic acid. Measurement is shown in Fig. 1. The culture with (-DAP) had a reduced growth by factor 3.21 compared to the control. The culture with 40 µM DAP added had a reduced growth by factor 2.30.

Fig. 1: Characterization of pGGA-dapAP-32cbtA-32cspD-32mraZ-30sulA. Cultures containing the growth inhibition module were inhibit with DAP and OD600 was measured for 7 h in 1 h intervals. Uninhibited cultures as well as DH5α were measured as positive controls.

Discussion

Upon addition of DAP (+DAP), cultures containing the growth inhibition module showed a growth comparable to the positive control, while the induced growth inhibited culture (-DAP) exhibited weak growth. While we expected the induced growth inhibited culture to not proliferate and not growth, a increase in growth rate was observable. Therefore we confirm that the growth inhibition module indeed reduces cell proliferation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]