Coding

Part:BBa_K2524000

Designed by: Holly Bowman   Group: iGEM17_Georgia_State   (2017-09-29)
Revision as of 02:34, 18 October 2018 by Jkelly38 (Talk | contribs)


Synthetic Human Chorionic Gonadotropin (HCG) Beta Subunit This part is an improvement of Part:BBa_K732001. Human Chorionic Gonadotropin (hCG) is the hormone made by chorionic cells in the fetal part of the placenta where it stimulates the ovaries to synthesize important steroids for the maintenance of pregnancy. It is a member of the beta chain glycoprotein hormone family. The glycoproteins are heterodimers consisting of an alpha subunit and beta subunit. The beta subunit determines bio-specificity. Six genes encode the hCG beta subunit which are inverted on chromosome 19q13.3 and is boarding the luteinizing hormone beta subunit gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

A

T--Georgia_State--hcgbetasubunit.jpg


Optimized primers for HCG for pSB1C3 Name: HCG New Prefix Primer 1 Sequence: agtcgaattcgcggccgcttctagATGGAAATGTTCCAGGGGCT Annealing Temperature: 56℃ Name: New HCG Suffix Sequence: GCAACTGCAGCGGCCGCTACTAGTATCACTGAGGAAGAATTGGGGTG Annealing Temperature: 64℃

Optimized primer for HCG for pGEX
Name: new hcg reverse for pGEX
Sequence: CGACGTACGGTCgcggccgcTCACTGAGGAAGAATTGGGGTG
Annealing Temperature: 70℃
Name: HCG for pGEX F
Sequence: cccaatttagaattcgATGGAAATGTTCCAGGGGCT
Annealing Temperature: 62℃



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Categories
Parameters
None