Composite
Part:BBa_K2787009
Designed by: Haoer Shi Group: iGEM18_ShanghaiTech (2018-10-09)
GFP under A2 orthogonal RBS with orthogonal 16S rRNA A2
The 16S rRNA A2 under constitutive promoter BBa_J23100(BBa_K2787006) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to justify the proper function of the ribosomal 16SRNA and RBS set A2.
Usage and Biology
a plasmid with GFP under control of A2 orthogonal RBS with A2 orthogonal 16s rRNA is constructed to test the orthogonality of B8 RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 797
Illegal NheI site found at 820 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1659
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 706
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Categories
Parameters
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