Composite
NOTMlcRE-A

Part:BBa_K2588024:Design

Designed by: Dominika Wawrzyniak, Daniel Wedemeyer   Group: iGEM18_Hamburg   (2018-10-05)
Revision as of 02:12, 18 October 2018 by BKlopprogge (Talk | contribs) (→‎Design Notes)

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3-methyl-1-butanol synthesis module, glucose-induced


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
    Illegal NheI site found at 167
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 7747
    Illegal XhoI site found at 570
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1328
    Illegal AgeI site found at 6485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7573
    Illegal SapI.rc site found at 4405


Design Notes

non

Source

Composite of BBa_K2588003, other standard registry parts, genes amplified by PCR from E. coli genomic dna, and synthetic DNA based on codon-optimized genes from Lactococcus lactis and saccharomyces cerevisiae.

References