Part:BBa_K2588008

Cell division inhibitor sulA

sulA is a protein from E. coli expressed in the SOS system to inhibit the cell division and is used in part of our growth inhibition module, BBa_K2588021

Usage and Biology

To inhibit the growth of E. coli, it is also necessary to inhibit the cell division. To realize this, we made use of the sulA gene. It expresses the sulA protein, which is found in E. coli as a part of the SOS system.¹ In presence of GTP and with an accumulating amount of sulA, the protein binds to the Z-ring forming FtsZ protein.² This leads to an inhibition of polymerization of FtsZ and consequently the Z-ring build up stops. Normally, cells use this process to prevent secretion of damaged DNA to daughter cells during cell division and to finally stops.³ In our project, we overexpressed the sulA with a modulated ribosome binding site as part of the growth inhibition module in E.coli.

References

1. Addinall, S. G., Bi, E. & Lutkenhaus, J. FtsZ ring formation in fts mutants. J. Bacteriol. 178, 3877–84 (1996).
2. Mukherjee, A., Cao, C. & Lutkenhaus, J. Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 95, 2885–90 (1998).
3. Bi, E. & Lutkenhaus, J. Analysis of ftsZ mutations that confer resistance to the cell division inhibitor SulA (SfiA). J. Bacteriol. 172, 5602–9 (1990).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

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