Reporter
mCherry

Part:BBa_J06504:Design

Designed by: ytwang   Group: iGEM2005   (2005-07-18)
Revision as of 01:50, 30 April 2006 by Registry (Talk | contribs)

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monomeric RFP optimized for bacteria


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR.


Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.


Source

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References

Files