Composite

Part:BBa_K2589001

Designed by: John Bechtel   Group: iGEM18_WLC-Milwaukee   (2018-10-10)
Revision as of 00:43, 18 October 2018 by JackBechtel (Talk | contribs)


Short His J-Protein (trc-promoter)

This part is a composite of the 2017 WLC-iGEM lambda phage Short J-Protein with N-Terminal His tag (BBa_K2452002) downstream of the trc-promoter (derived from pTrc99a) allowing for Isopropyl β-D-1-thiogalactopyranoside (IPTG) induction of the coding region.

Characterization

T--WLC-Milwaukee--Results_SDS_page_2_Med.jpg

T--WLC-Milwaukee--Results_Western_1_Med.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None