Composite

Part:BBa_K2587014

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-16)
Revision as of 00:09, 18 October 2018 by AnnaPhase (Talk | contribs)

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P_RBS_lasR_T

LasR is a component of the quorum sensing system and is used to start transciption of a target gene upon binding to the quorum sensing molecule AHL and the Plas promoter. The system originates from Pseudomonas aeruginosa but is codon optimized for E. coli here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 643
    Illegal SpeI site found at 802
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 643
    Illegal SpeI site found at 802
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 643
    Illegal SpeI site found at 802
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 643
    Illegal SpeI site found at 802
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 795


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Categories
Parameters
None