Regulatory

Part:BBa_K2560131:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-10-08)
Revision as of 23:59, 17 October 2018 by Henselt (Talk | contribs)


Phytobrick version of Promoter Dummy


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward oligo: CTCGGGAGCCCCTGGCGCCCCTTTACT

Reverse Oligo: CTCAAGTAAAGGGGCGCCAGGGGCTCC

Source

As Source DNA we used PYTK from the Dueber Toolbox. (Lee et al. 2015.)


Source

The promoter dummy was designed with a high GC-content to reduce propability of transcription initiation.